Rabbit Anti-ADAM17 antibody

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A disintegrin and metalloproteinase domain 17; ADAM 17; ADAM metallopeptidase domain 17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; MGC71942; Snake venom like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; Tumor Necrosis F

Cat:

SL4236R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human ADAM17:201-300/824<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti-ADAM17 (SL4236R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 68/93 kDObserved band size: 63/120 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-ADAM17 (SL4236R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 68/93 kDObserved band size: 93/120 kDBlank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-ADAM17 antibody(SL4236R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq].

Subunit:
Interacts with MAD2L1, MAPK14 and MUC1.

Subcellular Location:
Membrane.

Tissue Specificity:
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

Similarity:
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.

SWISS:
P78536

Gene ID:
6868

Database links:

Entrez Gene: 6868 Human

Entrez Gene: 11491 Mouse

Entrez Gene: 57027 Rat

Omim: 603639 Human

SwissProt: P78536 Human

SwissProt: Q9Z0F8 Mouse

SwissProt: Q9Z1K9 Rat

Unigene: 404914 Human

Unigene: 27681 Mouse

Unigene: 144585 Rat

Picture

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-ADAM17 (SL4236R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68/93 kD
Observed band size: 93/120 kD

Blank control: U937 (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-ADAM17 antibody(SL4236R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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