Rabbit Anti-MACC1 antibody | Sunlong Medical

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7A5; MACC1; MACC-1; MACC1_HUMAN; Metastasis associated in colon cancer 1; Metastasis-associated in colon cancer protein 1; Putative binding protein 7a5; SH3 domain-containing protein 7a5; SH3BP4L.

Cat:

SL4293R

Species Reactivity：

Human,Rat,(predicted: Mouse,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human MACC1:761-852/852

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:liver(mouse) Lysate at 40 ugPrimary: Anti-MACC1 (SL4293R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 94 kDObserved band size: 94 kDTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MACC1 Polyclonal Antibody, Unconjugated(SL4293R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MACC1) Polyclonal Antibody, Unconjugated (SL4293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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MACC1 is a key regulator of the hepatocyte growth factor(HGF; MIM 14489)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed19098908]).[supplied by OMIM].

Subunit:
Interacts with FASLG.

Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly found in the cytoplasm in non-metastasizing tumors.

Tissue Specificity:
Preferentially expressed in metastasizing tumors.

Similarity:
Contains 1 SH3 domain.

SWISS:
Q6ZN28

Gene ID:
346389

Database links:

Entrez Gene: 346389 Human

Entrez Gene: 238455 Mouse

Entrez Gene: 314539 Rat

Omim: 612646 Human

SwissProt: Q6ZN28 Human

Unigene: 598388 Human

Unigene: 31376 Mouse

Unigene: 81938 Rat

Picture

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MACC1 Polyclonal Antibody, Unconjugated(SL4293R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MACC1) Polyclonal Antibody, Unconjugated (SL4293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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