Rabbit Anti-TBRG1 antibody | Sunlong Medical

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NIAM; Nuclear interactor of ARF and Mdm2; Transforming growth factor beta regulator 1; TBRG1; TBRG-1; TBRG 1; Transforming growth factor beta regulator 1.

Cat:

SL4268R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human TBRG1:28-130/411

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Acts as a growth inhibitor. Can activate TP53/p53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability. There are two isoforms.

Function:
Acts as a growth inhibitor. Can activate p53/TP53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability.

Subunit:
Interacts with CDKN2A and MDM2.

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed at low levels in most tissues, with highest levels in pancreas, lung and liver. Expression is decreased in primary tumors including lung, liver, breast, pancreas and kidney carcinomas, chronic lymphocytic leukemia and diffuse large B-cell lymphoma.

Post-translational modifications:
Ubiquitinated; mediated by MDM2 and leading to its subsequent proteasomal degradation.

Similarity:
Belongs to the TBRG1 family.
Contains 1 FYR SLCterminal domain.
Contains 1 FYR N-terminal domain.

SWISS:
Q3YBR2

Gene ID:
84897

Database links:

Entrez Gene: 84897 Human

Entrez Gene: 21376 Mouse

Entrez Gene: 300521 Rat

Omim: 610614 Human

SwissProt: Q3YBR2 Human

SwissProt: Q3UB74 Mouse

SwissProt: Q5PQK8 Rat

Unigene: 436410 Human

Unigene: 28689 Mouse

Unigene: 129282 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBRG1) Polyclonal Antibody, Unconjugated (SL4268R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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