Rabbit Anti-GIG34 antibody | Sunlong Medical

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60S ribosomal protein L11; Cell growth inhibiting protein 34; Cell growth inhibiting protein34; CLL associated antigen KW 12; CLL associated antigen KW12; DBA 7; DBA7; GIG 34; GIG34; Ribosomal protein L11; RPL 11; RL11_HUMAN.

Cat:

SL5715R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human RPL11/GIG34:101-178/178

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Stomach (Mouse) Lysate at 40 ugLarge intestine (Mouse) Lysate at 40 ugPrimary: Anti-GIG34 (SL5715R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 20 kDObserved band size: 20 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti- GIG34 (SL5715R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 20 kDObserved band size: 20 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GIG34) Polyclonal Antibody, Unconjugated (SL5715R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010].

Function:
Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML (By similarity).

Subunit:
Interacts with PML and MDM2 (By similarity).

Subcellular Location:
Nucleus, nucleolus (By similarity).

DISEASE:
Diamond-Blackfan anemia 7 (DBA7) [MIM:612562]: A form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the ribosomal protein L5P family.

SWISS:
P62913

Gene ID:
6135

Database links:

Entrez Gene: 6135 Human

Entrez Gene: 67025 Mouse

Entrez Gene: 362631 Rat

Omim: 604175 Human

SwissProt: P62913 Human

SwissProt: Q9CXW4 Mouse

SwissProt: P62914 Rat

Unigene: 719951 Human

Unigene: 276856 Mouse

Unigene: 129770 Rat

Unigene: 14687 Rat

Picture

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- GIG34 (SL5715R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GIG34) Polyclonal Antibody, Unconjugated (SL5715R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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