Rabbit Anti-OSGIN1 antibody

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BDGI; Bone marrow stromal cell derived growth inhibitor; huOKL38; OKL 38; OKL38; OSGIN 1; Ovary kidney and liver protein 38; Oxidative stress induced growth inhibitor 1; Pregnancy induced growth inhibitor; Pregnancy induced growth inhibitor OKL38; OSGI1_H

Cat:

SL5723R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human OSGIN1:351-450/560

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-OSGIN1 (SL5723R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 61 kDObserved band size: 55 kDSample: MCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti-OSGIN1 (SL5723R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 61 kDObserved band size: 61 kDSample: Lane 1: Human HepG2 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-OSGIN1 (SL5723R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDaObserved band size: 61 kDaParaformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSGIN1) Polyclonal Antibody, Unconjugated (SL5723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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OSGIN1 (Oxidative stress-induced growth inhibitor 1) regulates the differentiation and proliferation of normal cells through the regulation of cell death.

Function:
Regulates the differentiation and proliferation of normal cells through the regulation of cell death.

Tissue Specificity:
Ubiquitous. Highest expression in the ovary, testis, kidney, and liver.

Similarity:
Belongs to the OKL38 family.

SWISS:
Q9UJX0

Gene ID:
29948

Database links:

Entrez Gene: 29948 Human

Entrez Gene: 71839 Mouse

Omim: 607975 Human

SwissProt: Q9UJX0 Human

Picture

Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-OSGIN1 (SL5723R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD

Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-OSGIN1 (SL5723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa

Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSGIN1) Polyclonal Antibody, Unconjugated (SL5723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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