Rabbit Anti-DSF4B antibody | Sunlong Medical

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Activator of S phase kinase like protein 1; ASK like protein 1; ASKL1; chifb; Chiffon homolog B; DBF 4B; DBF4 homolog B (S. cerevisiae); DBF4 homolog B; Dbf4 related factor 1; DRF1; Protein DBF4 homolog B; ZDBF1B; Zinc finger DBF type containing 1B; DBF4B

Cat:

SL5748R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human DBF4B/ASKL1:41-140/615

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela Cell (Human) Lysate at 30 ugPrimary: Anti-DSF4B (SL5748R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18kDObserved band size: 18kDSample: 293T Cell (Human) Lysate at 30 ugPrimary: Anti-DSF4B (SL5748R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18kDObserved band size: 18kDSample: U2os Cell (Human) Lysate at 30 ugPrimary: Anti-DSF4B (SL5748R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18kDObserved band size: 18kD

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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DSF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DSF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DSF4B localizes to the nucleus and its expression is cell cycle-regulated.

Function:
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DSF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.

Subunit:
Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DSF4/DSF4A or DSF4B. Such complexes are stable upon replication stress.

Subcellular Location:
Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.

Tissue Specificity:
Widely expressed. Highly expressed in testis.

Post-translational modifications:
Phosphorylated.

Similarity:
Contains 1 BRCT domain.
Contains 1 DSF4-type zinc finger.

SWISS:
Q8NFT6

Gene ID:
80174

Database links:

Entrez Gene: 80174 Human

Omim: 611661 Human

SwissProt: Q8NFT6 Human

Unigene: 369998 Human

Unigene: 728986 Human

Picture

Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-DSF4B (SL5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD

Sample: U2os Cell (Human) Lysate at 30 ug
Primary: Anti-DSF4B (SL5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD

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