Rabbit Anti-Lactoferrin antibody

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CKRX; GIG12; Growth inhibiting protein 12; HLF2; Homo sapiens chemokine receptor X; Kaliocin 1; Lactoferrin; Lactoferroxin A; Lactoferroxin B; Lactoferroxin C; Lactoferroxin-C; Lactotransferrin; LF; LTF; Neutrophil lactoferrin; Talalactoferrin alfa; Talal

Cat:

SL5810R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Lactoferrin:611-710/710

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lactoferrin protein (Human) 100 ngPrimary: Anti-Lactoferrin (SL5810R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 76 kDObserved band size: 85 kDSample: Breast milk (Human) Lysate at 40 ugPrimary: Anti-Lactoferrin (SL5810R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 76 kDObserved band size: 76 kDU-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(SL5810R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).Blank control: U-937. Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (SL5810R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.

Function:
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters.

Subunit:
Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1.

Subcellular Location:
Isoform 1: Secreted. Isoform DeltaLf: Cytoplasm. Nucleus.

Post-translational modifications:
Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391.

Similarity:
Belongs to the transferrin family.
Contains 2 transferrin-like domains.

SWISS:
P02788

Gene ID:
4057

Database links:

Entrez Gene: 4057 Human

Entrez Gene: 17002 Mouse

Entrez Gene: 301034 Rat

Omim: 150210 Human

SwissProt: P02788 Human

SwissProt: P08071 Mouse

Unigene: 529517 Human

Unigene: 282359 Mouse

Unigene: 218647 Rat

Picture

Sample: Breast milk (Human) Lysate at 40 ug
Primary: Anti-Lactoferrin (SL5810R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD

U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(SL5810R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).

Blank control: U-937.
Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (SL5810R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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