Rabbit Anti-AARE antibody | Sunlong Medical

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AARE; ACPH; Acyl peptide hydrolase; Acylamino acid releasing enzyme; Acylaminoacyl peptidase; APEH; APH; D3F15S2; D3S48E; DNF15S2; N acylaminoacyl peptide hydrolase; OPH; Oxidized protein hydrolase; ACPH_HUMAN.

Cat:

SL5983R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human AARE:101-200/732

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugHL60(Human) Cell Lysate at 30 ugU2OS(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugPrimary: Anti- AARE (SL5983R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 78 kDSample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti- AARE (SL5983R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 78 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AARE) Polyclonal Antibody, Unconjugated (SL5983R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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AARE (Acylamino-acid-releasing enzyme) is also known as Acyl-peptide hydrolase. It catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus corresponding to the protein are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Function:
This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.

Subunit:
Interacts with SUV420H1, SUV420H2 and USP4

Subcellular Location:
Nucleus.

Similarity:
Belongs to the retinoblastoma protein (RB) family.

SWISS:
P13798

Gene ID:
327

Database links:

Entrez Gene: 327 Human

Entrez Gene: 235606 Mouse

Entrez Gene: 24206 Rat

GenBank: 33875348 Human

Omim: 102645 Human

SwissProt: P13798 Human

SwissProt: Q8R146 Mouse

SwissProt: P13676 Rat

Unigene: 517969 Human

Unigene: 219639 Mouse

Unigene: 11057 Rat

Picture

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- AARE (SL5983R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 78 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AARE) Polyclonal Antibody, Unconjugated (SL5983R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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