Rabbit Anti-DAL1/EPB41L3 antibody

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Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN.

Cat:

SL6003R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human DAL1/EPB41L3:131-230/1087

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugBrain (Rat) Lysate at 40 ugU-87MG (Human) CellLysate at 30 ugHepG2 (Human) CellLysate at 30 ugPrimary: Anti-DAL1/EPB41L3 (SL6003R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 130-140 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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EPB41L3 is an actin-binding protein originally identified as a molecule whose expression is down-regulated in lung adenocarcinoma. EPB41L3 is a member of the protein 4.1 superfamily, which encompasses structural proteins that play important roles in membrane processes via interactions with actin, spectrin, and the cytoplasmic domains of integral membrane proteins. The gene for EPB41L3 is thought to function as a tumor suppressor and localizes within chromosomal region 18p11.3, which is affected by loss of heterozygosity (LOH) in various adult tumors.

Function:
Critical growth regulator in the pathogenesis of meningiomas.

Subunit:
Interacts with CADM1.

Subcellular Location:
Cytoplasm, cytoskeleton

Tissue Specificity:
Expressed at high levels in brain, with lower levels in kidney, intestine, and testis.

Similarity:
ontains 1 FERM domain.

SWISS:
Q9Y2J2

Gene ID:
23136

Database links:

Entrez Gene: 23136 Human

Entrez Gene: 13823 Mouse

Entrez Gene: 116724 Rat

Omim: 605331 Human

SwissProt: Q9Y2J2 Human

SwissProt: Q9WV92 Mouse

Unigene: 213394 Human

Unigene: 131135 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (SL6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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