Rabbit Anti-Wnt6 antibody | Sunlong Medical

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Protein Wnt-6 precursor; WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY, MEMBER 6; wnt 6; WNT6_HUMAN.

Cat:

SL6135R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human Wnt6:281-365/365

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-Wnt6 (SL6135R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 48 kDSample: MKN45(Human) Cell Lysate at 30 ugPrimary: Anti-Wnt6 (SL6135R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 42 kDTissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated(SL6135R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Wnt6 is a member of the WNT protein family. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt6 is overexpressed in cervical and breast cell carcinomas and is co-expressed with Wnt10a in colorectal cancer cell lines.

Subunit:
Interacts with PORCN.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Similarity:
Belongs to the Wnt family.

SWISS:
Q9Y6F9

Gene ID:
7475

Database links:

Entrez Gene: 7475 Human

Entrez Gene: 22420 Mouse

Entrez Gene: 316526 Rat

Omim: 604663 Human

SwissProt: Q9Y6F9 Human

SwissProt: P22727 Mouse

Unigene: 29764 Human

Unigene: 268282 Mouse

Picture

Sample:
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-Wnt6 (SL6135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 42 kD

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated(SL6135R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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