Rabbit Anti-THRA1+2 antibody

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Thyroid Hormone Receptor alpha 1+2; THRA1+THRA2; AR7; Avian erythroblastic leukemia viral (v erb a) oncogene homolog; c ERBA 1; c ERBA ALPHA 2; C erbA alpha; c-erbA-1; C-erbA-alpha; cerbA protein; cerbAalpha; EAR 7; EAR 7.1; EAR 7.2; EAR-7; EAR7; ERB T 1;

Cat:

SL6222R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Zebrafish,Sheep,Cat,)

Immunogen：

KLH conjugated synthetic peptide derived from human THRA1 and THRA2:101-200/490

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Stomach (Mouse) Lysate at 40 ugPrimary: Anti- THRA1+2 (SL6222R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 53 kDSample: Stomach (Mouse) Lysate at 40 ugPrimary: Anti-THRA1+2 (SL6222R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDParaformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THRA1+2) Polyclonal Antibody, Unconjugated (SL6222R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

Function:
Nuclear hormone receptor. High affinity receptor for triiodothyronine.

Subunit:
Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D (By similarity). Interacts with AKAP13.

Subcellular Location:
Nucleus.

DISEASE:
Defects in THRA are the cause of congenital hypothyroidism non-goitrous type 6 (CHNG6) [MIM:614450]. A disease characterized by growth retardation, developmental retardation, skeletal dysplasia, borderline low thyroxine levels and high triiodothyronine levels. There is differential sensitivity to thyroid hormone action, with retention of hormone responsiveness in the hypothalamic pituitary axis and liver but skeletal, gastrointestinal, and myocardial resistance.

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P10827

Gene ID:
7067

Database links:

Entrez Gene: 7067 Human

Entrez Gene: 21833 Mouse

Entrez Gene: 81812 Rat

Omim: 190120 Human

SwissProt: P10827 Human

SwissProt: P63058 Mouse

SwissProt: O97716 Pig

SwissProt: P63059 Rat

Unigene: 724 Human

Unigene: 265917 Mouse

Unigene: 442648 Mouse

Unigene: 11307 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THRA1+2) Polyclonal Antibody, Unconjugated (SL6222R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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