MAGEB1 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21.
Tissue Specificity:
Expressed only in testis.
Similarity:
Contains 1 MAGE domain.
SWISS:
Q96M61
Gene ID:
286514
Database links:
Entrez Gene: 286514 Human
SwissProt: Q96M61 Human
Unigene: 350683 Human
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Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-MAGEB1 (SL6821R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-MAGEB1 (SL6821R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Tissue/cell: Dog testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-MAGEB1 Polyclonal Antibody, Unconjugated(SL6821R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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