Rabbit Anti-CARD15/NOD2 antibody

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ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; CD; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; LRR-containing protein; Nod2; NOD2 prot

Cat:

SL7084R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CARD15:251-350/1040

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-NOD2 (SL7084R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDSample: A549(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-NOD2 (SL7084R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 114 kDObserved band size: 114 kDBlank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody(SL7084R-/AF488 ), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) . EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.

Subunit:
Binds to RIPK2/RICK by CARD-CARD interaction.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Monocytes-specific.

Similarity:
Contains 2 CARD domains.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.

SWISS:
Q9HC29

Gene ID:
64127

Database links:

Entrez Gene: 64127 Human

Entrez Gene: 257632 Mouse

Entrez Gene: 291912 Rat

Omim: 605956 Human

SwissProt: Q9HC29 Human

SwissProt: Q8K3Z0 Mouse

Unigene: 592072 Human

Unigene: 222633 Mouse

Unigene: 218600 Rat

CARD15与家族性肠黏膜克罗恩病有关。 Picture

Sample:
A549(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-NOD2 (SL7084R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD

Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody(SL7084R-/AF488 ), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );

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