Rabbit Anti-DNase I antibody

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RNASE1; Dornase alfa; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease1; DeoxyribonucleaseI; DNASE 1; DNase I lysosomal; DNASE1; DNaseI; DNL 1; DNL1; DRNI; Human urine deoxyribonuclease I; DNAS1_HUMAN.

Cat:

SL7651R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Pig,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human DNase I:101-200/282

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MOLT-4(Human) Cell Lysate at 30 ugPrimary: Anti-DNase I (SL7651R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDSample: MKN45(Human) Cell Lysate at 30 ugPrimary: Anti-DNase I (SL7651R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kD Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNase I) Polyclonal Antibody, Unconjugated (SL7651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.

Subcellular Location:
Secreted. Nucleus envelope.

Tissue Specificity:
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

Similarity:
Belongs to the DNase I family.

SWISS:
P24855

Gene ID:
1773

Database links:

Entrez Gene: 1773 Human

Omim: 125505 Human

SwissProt: P24855 Human

Unigene: 629638 Human

Picture

Sample:
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti-DNase I (SL7651R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNase I) Polyclonal Antibody, Unconjugated (SL7651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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