Rabbit Anti-Cyp2J3 antibody

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CP2J3_RAT; cytochrome P450, family 2, subfamily j, polypeptide 3; cytochrome P450 monooxygenase; Cyp2j9; cytochrome P450, family 2, subfamily j, polypeptide 9; cytochrome P450, family 2, subfamily j, polypeptide 3; CYPIIJ3; cytochrome P450 2J3.

Cat:

SL2123R

Species Reactivity：

Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from rat Cyp2-j3:401-502/502

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Rat) Lysate at 40 ugBrain (Rat) Lysate at 40 ugPrimary: Anti-Cyp2J3 (SL2123R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-Cyp2J3 (SL2123R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDTissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated(SL2123R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016].

Function:
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation mainly to 14,15-, 11,12-, and 8,9-epoxyeicosatrienoic acids (EET). It also acts as an omega-1-hydroxylase by metabolizing arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-OH-AA).

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue Specificity:
Abundantly expressed in heart and liver.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
P51590

Gene ID:
313375

Database links:

Picture

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Cyp2J3 (SL2123R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated(SL2123R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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