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Home > Product > Antibody > Rabbit Anti-DSN1 antibody
C20orf172; dJ469A13.2; DSN1; DSN1, MIND kinetochore complex component, homolog; DSN1, MIND kinetochore complex component, homolog (S. cerevisiae); DSN1_HUMAN; FLJ13346; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kine
Cat:
SL7796R
Species Reactivity：
Mouse,Rat,(predicted: Human,Dog,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human DSN1:161-250/356
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Bone (Mouse) Lysate at 40 ugPrimary: Anti-DSN1 (SL7796R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40kDObserved band size: 40kDParaformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1) Polyclonal Antibody, Unconjugated (SL7796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1) Polyclonal Antibody, Unconjugated (SL7796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


This gene encodes a kinetochore protein that functions aspart of the minichromosome instability-12 centromere complex. Theencoded protein is required for proper kinetochore assembly andprogression through the cell cycle. Alternative splicing results inmultiple transcript variants. [provided by RefSeq, Feb 2009].

Function:
Part of the MIS12 complex which is required for normalchromosome alignment and segregation and kinetochore formationduring mitosis.

Subunit:
Component of the MIS12 complex composed of MIS12, DSN1,NSL1 and PMF1. Also interacts with CASC5, CBX3 and CBX5.

Subcellular Location:
Nucleus. Chromosome, centromere,kinetochore.

SWISS:
Q9H410

Gene ID:
79196

Database links:

Entrez Gene: 79196 Human

Omim: 609175 Human

SwissProt: Q9H410 Human

Unigene: 632268 Human



Picture

Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-DSN1 (SL7796R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40kD
Observed band size: 40kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1) Polyclonal Antibody, Unconjugated (SL7796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1) Polyclonal Antibody, Unconjugated (SL7796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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