Home > Product > Antibody > Rabbit Anti-BMP9 antibody
BMP 9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2; GDF-2; growth differentiation factor 2; GDF2_HUMAN.
Cat:
SL6909R
Species Reactivity:
Human,Mouse,Rat,(predicted: Rabbit,)
Immunogen:
KLH conjugated synthetic peptide derived from human BMP9:351-429/429
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-BMP9 (SL6909R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/47 kDObserved band size: 50 kDSample: HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-BMP9 (SL6909R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/47 kDObserved band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP9) Polyclonal Antibody, Unconjugated (SL6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
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Product PDFs
Datasheet:


The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. [provided by RefSeq, Jul 2008].

Function:
Could be involved in bone formation.

Subunit:
Homodimer; disulfide-linked.

Subcellular Location:
Secreted.

Similarity:
Belongs to the TGF-beta family.

SWISS:
Q9UK05

Gene ID:
2658

Database links:

Entrez Gene: 2658 Human

Entrez Gene: 12165 Mouse

Entrez Gene: 290921 Rat

Omim: 605120 Human

SwissProt: Q9UK05 Human

SwissProt: Q9WV56 Mouse

Unigene: 218418 Rat



Picture

Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-BMP9 (SL6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-BMP9 (SL6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP9) Polyclonal Antibody, Unconjugated (SL6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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