Rabbit Anti-CTDSPL antibody

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Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; CTD small phosphatase-like protein; CTDSL_HUMAN; CTDSP-like; Ctdspl; hYA22; NIF-like protein; NLI-interacting factor 1; Nuclear LIM interactor-interacting factor 1; Protein YA22;

Cat:

SL8241R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human CTDSPL:175-276/276

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugPrimary: Anti-CTDSPL (SL8241R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDParaformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTDSPL) Polyclonal Antibody, Unconjugated (SL8241R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-CTDSPL antibody (SL8241) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the SLCterminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

Function:
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the SLCterminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

Subunit:
Monomer. Interacts with REST.

Subcellular Location:
Nucleus (By similarity).

Tissue Specificity:
Expression is restricted to non-neuronal tissues.

Similarity:
Contains 1 FCP1 homology domain.

SWISS:
O15194

Gene ID:
10217

Database links:

Entrez Gene: 10217 Human

Entrez Gene: 69274 Mouse

Entrez Gene: 301056 Rat

Omim: 608592 Human

SwissProt: O15194 Human

SwissProt: P58465 Mouse

SwissProt: Q5XIK8 Rat

Unigene: 475963 Human

Unigene: 439928 Mouse

Unigene: 37030 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTDSPL) Polyclonal Antibody, Unconjugated (SL8241R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-CTDSPL antibody (SL8241)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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