Rabbit Anti-UBE2Q1 antibody

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Galactosyl transferase associated protein; GTAP; NICE 5; NICE5; PRO3094; Protein NICE 5; Protein NICE-5; UB2Q1_HUMAN; UBE2Q; Ube2q1; Ubiquitin carrier protein Q1; Ubiquitin conjugating enzyme E2 Q1; Ubiquitin conjugating enzyme E2Q (putative) 1; Ubiquitin

Cat:

SL8383R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human UBE2Q1:321-422/422

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000Flow-Cyt=2μg /Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

cells:mouse splenocyteConcentration: 2μg/10^6 cells, 4℃ incubate 30min.Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (SL8383R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (UBE1s), ubiquitin-conjugating enzymes (UBE2s), and ubiquitin-protein ligases (UBE3s). When ubiquitin is activated by a UBE1, it is transferred to the cysteine residue on a UBE2. UBE2 then binds a UBE3, which transfers the ubiquitin from the UBE2 cysteine to a lysine residue on the target protein. Ubiquitin-conjugating enzyme E2 Q1 (UBE2Q1), also known as ubiquitin-protein ligase Q1 or ubiquitin carrier protein Q1, is an 422 amino acid protein belonging to the ubiquitin-conjugating enzyme (UBE2) family. Two named isoforms of UBE2Q1 exist as a result of alternative splicing events.

Function:
Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

SWISS:
Q7Z7E8

Gene ID:
55585

Database links:
UniProtKB/Swiss-Prot: Q7Z7E8.1

Picture

cells:mouse splenocyte
Concentration: 2μg/10^6 cells, 4℃ incubate 30min.

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (SL8383R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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