This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011].
Function:
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Subunit:
Homodimer upon hormone binding. Interacts with SMARCA1. Interacts with GH1. Interacts with CSH. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Isoform 7: Secreted.
Tissue Specificity:
Expressed in breast, placenta, kidney, liver and pancreas.
Similarity:
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
SWISS:
P16471
Gene ID:
5618
Database links:
Entrez Gene: 100053793 Horse
Entrez Gene: 5618 Human
Entrez Gene: 414916 Pig
Entrez Gene: 24684 Rat
Omim: 176761 Human
SwissProt: P16471 Human
SwissProt: Q6JTA8 Pig
SwissProt: P14787 Rabbit
SwissProt: P05710 Rat
SwissProt: O46561 Sheep
Unigene: 368587 Human
Unigene: 9757 Rat
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-PRLR (SL6445R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PRLR Polyclonal Antibody, Unconjugated(SL6445R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRLR) Polyclonal Antibody, Unconjugated (SL6445R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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