This gene encodes a peripheral membrane protein and is located in a region of chromosome 10q that contains a segmental duplication. This copy of the gene is full-length and is in the telomeric duplicated region. Two other more centromerically proximal copies of the gene are partial and may represent pseudogenes. This full-length gene appears to function in the establishment and maintenance of cell polarization. The protein is recruited to cell-cell junctions in an E-cadherin-dependent manner, and is selectively localized at the basolateral membrane in polarized epithelial cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2009]
Function:
May play a role in the regulation of tight junction formation. Binds phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2).
Subunit:
Interacts (via the PDZ 2 domain) with CTNND2 (via the extreme SLCterminus). Interacts (via the PDZ 2 domain) with PKP4 (via the extreme SLCterminus); this interaction directed FRMPD2 to the basolateral membranes. Interacts (via the PDZ 2 domain) with ARVCF (via the extreme SLCterminus).
Subcellular Location:
Cytoplasm (Potential). Basolateral cell membrane. Cell junction, tight junction. Note=Colocalizes with CTNNB1, CDH1 and PKP4 at the basolateral membrane. Colocalizes with TJP1 at tight junctions. Its recruitment to cell-cell contacts requires CDH1.
Tissue Specificity:
Expressed in epithelial cells.
Similarity:
Contains 1 FERM domain.
Contains 1 KIND domain.
Contains 3 PDZ (DHR) domains.
SWISS:
Q68DX3
Gene ID:
143162
Database links:
Entrez Gene: 143162 Human
SwissProt: Q68DX3 Human
Unigene: 664786 Human
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- FRMPD2 (SL9038R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 144 kD
Observed band size: 144 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FRMPD2) Polyclonal Antibody, Unconjugated (SL9038R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FRMPD2) Polyclonal Antibody, Unconjugated (SL9038R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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