Home > Product > Antibody > Rabbit Anti-AIM1L antibody
Absent in melanoma 1 like; Absent in melanoma 1-like protein; AIM1L; AIM1L_HUMAN; Beta/gamma crystallin domain-containing protein 2; CRYBG2; MGC183649; RP11-569G9.5; RP23-314D24.4; DKFZp434L1713; FLJ10040; FLJ38020.
Cat:
SL9093R
Species Reactivity:
Human,Mouse,(predicted: Rat,Dog,Cow,)
Immunogen:
KLH conjugated synthetic peptide derived from human AIM1L:51-150/616
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti- AIM1L (SL9093R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 69 kDObserved band size: 69 kDSample: A431(Human) Cell Lysate at 30 ugPrimary: Anti- AIM1L (SL9093R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 69 kDObserved band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIM1L) Polyclonal Antibody, Unconjugated (SL9093R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- AIM1L (SL9093R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- AIM1L (SL9093R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIM1L) Polyclonal Antibody, Unconjugated (SL9093R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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