Members of the class-III pyridoxal-phosphate-dependent aminotransferase family, such as AGXT2, catalyze the conversion of glyoxylate to glycine using L-alanine as the amino donor. AGXT2 protects from asymmetric dimethylarginine (ADMA)-induced inhibition in nitric oxide (NO) production. Elevated blood concentrations of ADMA, a methyl derivate of the amino acid arginine and an endogenous inhibitor of nitric oxide (NO) synthase, is produced by the physiological degradation of methylated proteins and is found in association with diabetes, hypertension, congestive heart failure and atherosclerosis. AGXT2L2 (alanine-glyoxylate aminotransferase 2-like 2) is a 450 amino acid pyridoxal phosphate that exists as a homotetramer. Belonging to the class-III pyridoxal-phosphate-dependent aminotransferase family, AGXT2L2 localizes to the mitochondria and exists as three alternatively spliced isoforms. Encoded by a gene located on human chromosome 5q35.3, AGXT2L2 may have similar functions as AGXT2.
Function:
Catalyzes the pyridoxal-phosphate-dependent breakdown of 5-phosphohydroxy-L-lysine, converting it to ammonia, inorganic phosphate and 2-aminoadipate semialdehyde.
Subunit:
Homotetramer.
Subcellular Location:
Mitochondrial
Similarity:
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
SWISS:
Q8IUZ5
Gene ID:
85007
Database links:
Entrez Gene: 85007 Human
Entrez Gene: 72947 Mouse
Entrez Gene: 100169747 Rat
Omim: 614683 Human
SwissProt: Q8IUZ5 Human
SwissProt: Q8R1K4 Mouse
Unigene: 248746 Human
Unigene: 490267 Mouse
Unigene: 217734 Rat
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Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-AGXT2L2 (SL9095R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
Sample: Heart (mouse) Lysate at 40 ug
Primary: Anti- AGXT2L2 (SL9095R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-AGXT2L2 Polyclonal Antibody, Unconjugated(SL9095R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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