Rabbit Anti-C21orf2 antibody

Home > Product > Antibody > Rabbit Anti-C21orf2 antibody

A2 antibody C21orf HUMF09G8.5; Chromosome 21 open reading frame 2; Hypothetical protein LOC755; Nuclear encoded mitochondrial protein cDNA A2 YF5; Uncharacterized protein C21orf2; YF 5; YF5; YF5/A2 antibody.

Cat:

SL9977R

Species Reactivity：

Human,Rat,(predicted: Mouse,)

Immunogen：

KLH conjugated synthetic peptide derived from human C21orf2:1-100/256

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549(Human) Cell Lysate at 30 ugPrimary: Anti-C21orf2 (SL9977R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 27 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C21orf2) Polyclonal Antibody, Unconjugated (SL9977R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-C21orf2 Polyclonal Antibody, Unconjugated(SL9977R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The smallest of the human chromosomes, 21 makes up about 1.5% of the human genome. Chromosome 21 contains nearly 300 genes and 47 million base pairs. Down syndrome, also known as trisomy 21, is the disease most commonly associated with chromosome 21. Alzheimer's disease, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis are also associated with chromosome 21. Translocations are found to occur between chromosome 21 and 8, and chromosome 21 and 12, in certain leukemias. The C21orf2 gene product has been provisionally designated C21orf2 pending further characterization.

Function:
May play roles in cilia formation and/or maintenance (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization.

Similarity:
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
To C.elegans F09G8.5.

SWISS:
O43822

Gene ID:
755

Database links:

Entrez Gene: 755 Human

Entrez Gene: 67884 Mouse

Omim: 603191 Human

SwissProt: O43822 Human

SwissProt: Q8C6G1 Mouse

Unigene: 517331 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C21orf2) Polyclonal Antibody, Unconjugated (SL9977R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-C21orf2 Polyclonal Antibody, Unconjugated(SL9977R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall