Rabbit Anti-ILVBL antibody | Sunlong Medical

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Acetolactate synthase homolog; Acetolactate synthase like protein; Acetolactate synthase-like protein; AHAS; ILV2H; 209L8; IlvB (bacterial acetolactate synthase) like; IlvB like protein; IlvB-like protein; Ilvbl; ILVBL_HUMAN.

Cat:

SL11014R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human ILVBL:301-400/632

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=3ug/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hcclm3(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugPrimary: Anti- ILVBL (SL11014R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 68 kDObserved band size: 68 kDSample: HepG2 Cell (Human) Lysate at 30 ugPrimary: Anti-ILVBL (SL11014R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 68kDObserved band size: 68kDBlank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (SL11014R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ILVBL is a 632 amino acid single-pass membrane protein that belongs to the TPP enzyme family. Expressed in the majority of tissues, ILVBL has the highest level of expression in heart, pancreas and placenta. ILVBL is highly homologous to several bacterial enzymes, including the B isozyme of the large catalytic subunit of E. coli acetohydroxy-acid synthase (AHAS) and the oxalyl-coA decarboxylase of O. formigenes, that utilize thiamine pyrophosphate as a cofactor. ILVBL binds one magnesium ion and one thiamine pyrophosphate per subunit, and may catalyze the initial step in branched-chain amino acid biosynthesis. The gene encoding ILVBL maps to human chromosome 19p13.12 and mouse chromosome 10 C1.

Subcellular Location:
Membrane.

Tissue Specificity:
Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.

Similarity:
Belongs to the TPP enzyme family.

SWISS:
A1L0T0

Gene ID:
10994

Database links:

Entrez Gene: 10994 Human

Omim: 605770 Human

SwissProt: A1L0T0 Human

Unigene: 78176 Human

Picture

Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-ILVBL (SL11014R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-ILVBL antibody (SL11014R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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