Rabbit Anti-Nova1 antibody | Sunlong Medical

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Neuro oncological ventral antigen 1; Neuro-oncological ventral antigen 1; NOVA 1; Nova-1; NOVA1_HUMAN; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA binding protein Nova 1; RNA-binding protein Nova-1; Ventral neuron specific protein 1; Vent

Cat:

SL11324R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human Nova1:411-510/510

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-Nova1 (SL11324R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDSample: DU145(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugPrimary: Anti- Nova1 (SL11324R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 50 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Nova1 (SL11324R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (SL11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (SL11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nova-1 and Nova-2 are members of a superfamily of protein regulators of RNA metabolism in neurons. Both are nuclear RNA binding proteins with K homology motifs, conserved protein sequences which bind to RNA (1,2). Nova proteins, normally sequestered in the central nervous system, are expressed by systemic tumors in patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA) (3,4). Nova-1 is expressed in the hindbrain and ventral spinal cord and Nova-2 is expressed in the neocortex and hippocampus (4). Nova-1 is necessary for regulating neuron-specific alternative splicing of the glycine receptor Alpha2 pre-mRNA (5).

Function:
May regulate RNA splicing or metabolism in a specific subset of developing neurons.

Subunit:
Interacts with PTBP2; the interaction is direct (By similarity).

Subcellular Location:
Nucleus.

Tissue Specificity:
Brain.

Similarity:
Contains 3 KH domains.

SWISS:
P51513

Gene ID:
4857

Database links:

Entrez Gene: 4857 Human

Entrez Gene: 664883 Mouse

Entrez Gene: 298992 Rat

Omim: 602157 Human

SwissProt: Q2PFW9 Cynomolgus monkey

SwissProt: P51513 Human

SwissProt: Q9JKN6 Mouse

SwissProt: Q80WA4 Rat

Picture

Sample:
DU145(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Nova1 (SL11324R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 50 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Nova1 (SL11324R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (SL11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nova1) Polyclonal Antibody, Unconjugated (SL11324R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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