Synaptic vesicle recycling involves numerous proteins that contribute to the formation and trafficking of the SNARE complexes throughout the cell. Synaptoporin, also designated synaptophysin 2, is an integral membrane protein of small synaptic vesicles that belongs to the synaptophysin/synaptobrevin family. Synaptoporin is highly homologus to Synaptophysin 1 and both Synaptoporin and synaptophysin 1 contain four transmembrane domains and a short cytoplasmic tail. The Synaptoporin protein also contains one MARVEL domain, a membrane-associating domain found in lipid-associating proteins, and displays calcium-binding activity which may be localized to its cytoplasmic tail. Syntaphilin, synaptophysin and Synaptoporin regulate the formation of the vesicles by competing with components of the SNARE complexes to respectively inhibit either the assembly or the secretion of the synaptic vesicles.
Function:
Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein.
Subcellular Location:
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
Similarity:
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.
SWISS:
Q8TBG9
Gene ID:
132204
Database links:
Entrez Gene: 132204 Human
Entrez Gene: 14403 Mouse
Entrez Gene: 66030 Rat
SwissProt: Q8TBG9 Human
SwissProt: Q8BGN8 Mouse
SwissProt: P22831 Rat
Unigene: 648668 Human
Unigene: 317515 Mouse
Unigene: 211986 Rat
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Sample:
Bulbus olfactorius (Rat) Lysate at 40 ug
Primary: Anti-Synaptoporin (SL11367R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (SL11367R) at 1:800 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(SL11367R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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