(mouse/tissue)Cerebellum lysates probed with p-Tau(Thr231) Polyclonal Antibody, Unconjugated (Catalog #SL10106R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #926-32211) at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Tau (Thr548)) Polyclonal Antibody, Unconjugated (SL10106R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.