CRBN is a 442 amino acid protein which is highly concentrated in human brain tissue. CRBN functions are thought to be related to energy metabolism, learning and memory. Localized to the cytoplasm, CRBN acts as a protease in mitochondria and is thought to regulate the assembly of KCNT1, as well as the surface expression of KCNT1 in brain regions known to affect memory and learning, such as the hippocampus. The gene encoding CRBN belongs to a family of ATP-dependent lon proteases that play a role in membrane trafficking and proteolysis. Defects in the CRBN gene are associated with mild mental retardation.
Function:
Protein cereblon modulates cell surface expression of KCNT1 and may be involved in memory and learning. It is highly expressed in brain and defects in CRBN are the cause of non syndromic mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. Although it contains a Lon domain also found in proteases of the peptidase S16 family, it does not contain the ATP binding and catalytic domains, suggesting that it has no protease activity.
Subunit:
Interacts with KCNT1 (By similarity). Component of a DCX (DDB1-CUL4-X-box) protein ligase complex, at least composed of CRBN, CUL4A, DDB1 and RBX1.
Subcellular Location:
Cytoplasm. Nucleus. Membrane; Peripheral membrane protein
Tissue Specificity:
Widely expressed. Highly expressed in brain.
Post-translational modifications:
Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
DISEASE:
Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
Similarity:
Belongs to the CRBN family.
Contains 1 Lon domain.
SWISS:
Q96SW2
Gene ID:
51185
Database links:
Entrez Gene: 51185 Human
Entrez Gene: 58799 Mouse
Entrez Gene: 297498 Rat
Entrez Gene: 445491 Zebrafish
GenBank: NM_016302.2 Human
Omim: 607417 Human
SwissProt: Q96SW2 Human
SwissProt: Q8C7D2 Mouse
SwissProt: Q56AP7 Rat
SwissProt: Q68EH9 Zebrafish
Unigene: 18925 Human
Unigene: 58085 Mouse
Unigene: 195259 Rat
Unigene: 84847 Zebrafish
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRBN) Polyclonal Antibody, Unconjugated (SL11716R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CRBN Polyclonal Antibody, Unconjugated(SL11716R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CRBN Polyclonal Antibody, Unconjugated(SL11716R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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