Rabbit Anti-Puratrophin 1 antibody

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ARHGEF44; PH domain-containing family G member 4; PKHG4_HUMAN; Pleckstrin homology domain containing family G (with RhoGef domain) member 4; member 4; Pleckstrin homology domain-containing family G member 4; PLEKHG 4; PLEKHG4; PRTPHN 1; PRTPHN1; Puratroph

Cat:

SL11747R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Puratrophin 1/PLEKHG4:945-1045/1191

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- Puratrophin 1 (SL11747R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 131 kDObserved band size: 131 kDParaformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Puratrophin 1) Polyclonal Antibody, Unconjugated (SL11747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Puratrophin 1) Polyclonal Antibody, Unconjugated (SL11747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene contains multiple domains suggestive of a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Mutations in this gene are associated with spinocerebellar ataxia 16q22-linked. Several alternatively spliced transcript variants, differing only in the 5' UTR, or encoding a different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.

Tissue Specificity:
Expressed in kidney, Leydig cells in thetestis, epithelial cells in the prostate gland and Langerhans isletin the pancreas. Isoform 1 and isoform 3 are strongly expressed inPurkinje cells and to a lower extent in other neurons (at proteinlevel). Widely expressed at low levels. More strongly expressed intestis and pancreas.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.

SWISS:
Q58EX7

Gene ID:
25894

Database links:

Entrez Gene: 25894 Human

Omim: 609526 Human

SwissProt: Q58EX7 Human

Unigene: 188781 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Puratrophin 1) Polyclonal Antibody, Unconjugated (SL11747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Puratrophin 1) Polyclonal Antibody, Unconjugated (SL11747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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