Rabbit Anti-E cadherin antibody

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E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion mo

Cat:

SL10009R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human E-cadherin:401-500/882<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-E cadherin (SL10009R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 125 kDObserved band size: 125 kDSample: HT29(Human) Cell Lysate at 30 ugPSLC3(Human) Cell Lysate at 30 ugPrimary: Anti-E cadherin (SL10009R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 20/80 kDObserved band size: 130 kD Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit:
Homodimer.

Subcellular Location:
Cell junction. Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Non-neural epithelial tissues.

Post-translational modifications:
Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) . An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.

Similarity:
Contains 5 cadherin domains.

SWISS:
P12830

Gene ID:
999

Database links:

Entrez Gene: 999 Human

Entrez Gene: 12550 Mouse

Entrez Gene: 83502 Rat

Entrez Gene: 282637 Cow

Entrez Gene: 442858 Dog

Omim: 192090 Human

SwissProt: Q6R8F2 Cow

SwissProt: P12830 Human

SwissProt: P01963 Mouse

SwissProt: Q9R0T4 Rat

Unigene: 461086 Human

Unigene: 35605 Mouse

Unigene: 1303 Rat

Picture

Sample:
HT29(Human) Cell Lysate at 30 ug
PSLC3(Human) Cell Lysate at 30 ug
Primary: Anti-E cadherin (SL10009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/80 kD
Observed band size: 130 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Specific References (39) | SL10009R has been referenced in 39 publications.

[IF=3.388] Liu H et al. Circular RNA has_circ_000644 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis. Cell Signal. 2020 Jun;70:109595. WB ; human.

PubMed:32151659

[IF=5.572] Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332. WB ; Human.

PubMed:32473523

[IF=2.073] Jin Dai. et al. NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway. Cell Biochem Biophys. 2021 Mar;79(1):123-131 WB ; Human.

PubMed:33009998

[IF=1.841] Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223 WB ; Human.

PubMed:33957574

[IF=4.101] Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11 WB ; Human.

PubMed:34958109

[IF=10.435] Shi, Yesi. et al. Biomimetic nanoparticles blocking autophagy for enhanced chemotherapy and metastasis inhibition via reversing focal adhesion disassembly. J Nanobiotechnol. 2021 Dec;19(1):1-17 WB ; Human.

PubMed:34952594

[IF=4.192] Chen D et al. Sodium Propionate Attenuates the Lipopolysaccharide-Induced Epithelial–Mesenchymal Transition via the PI3K/Akt/mTOR Signaling Pathway. J Agric Food Chem. 2020 Jun 17;68(24):6554-6563. IHC ; Mouse.

PubMed:32452677

[IF=10.679] Pan J et al. lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling. Mol Cancer. 2020 Jan 15;19(1):9. WB ; Human.

PubMed:31941509

[IF=3.06] Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. WB ; Human.

PubMed:31906574

[IF=5.714] Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. WB ; Human.

PubMed:3316991

[IF=3.775] Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751. WB ; Rat.

PubMed:31390532

[IF=2.705] Zhou S et al. Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Biochem Biophys Res Commun. 2019 Jun 30;514(3):893-900. IHSLCP,ICF&WB ; Human.

PubMed:31084933

[IF=3.249] Lin X et al. TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway.(2018)J Cancer;Oct 31;9(23):4430-4439. ICC ; Human.

PubMed:30519349

[IF=2.092] Guo F et al. miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells.(2018) Clin Exp Pharmacol Physiol. Oct 24. WB ;

PubMed:30353914

[IF=4.357] Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158 WB ; Human.

PubMed:26702618

[IF=1.28] Jinquan Cai Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. (2013) Exp.Ther.Me. 6:1489-1493 IHSLCP ; Human.

PubMed:24255679

[IF=2.81] Hu et al. Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition. (2016) PLoS.On. 11:e0153409 WB ; Mouse.

PubMed:27088510

[IF=6.1] Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017). WB ; Human.

PubMed:28901751

[IF=7.43] Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10. WB ; Human.

PubMed:28193222

[IF=4.069] Geng X et al. Role of ZIP8 in regulating cell morphology and NF-κB/Snail2 signaling.Metallomics. 2018 Jul 18;10(7):953-964. ICF ; Mouse.

PubMed:29927450

[IF=4.36] Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937 WB ; Human.

PubMed:34958876

[IF=5.211] Jin, Meiyuan. et al. MicroRNA-3935 promotes human trophoblast cell epithelial-mesenchymal transition through tumor necrosis factor receptor-associated factor 6/regulator of G protein signaling 2 axis. Reprod Biol Endocrin. 2021 Dec;19(1):1-15 WB ; human.

PubMed:34493304

[IF=5.682] Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464–18481 WB,IHC ; Mouse.

PubMed:34260413

[IF=4.101] Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13 WB ;

PubMed:34132357

[IF=2.349] Shunichiro Hanai. et al. Hypoxia-induced thyroid hormone receptor expression regulates cell-cycle progression in renal tubule epithelial cells. 2021 Jun 08 IHC ; Human.

PubMed:34108302

[IF=4.147] Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773 IHC ; Human.

PubMed:33249322

[IF=2.942] Shi M et al. MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/β-Catenin signalling. Biosci Rep. 2020 Jun 26;40(6):BSR20192794. WB&ICF ; Rat.

PubMed:32484208

[IF=3.04] Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. WB ; Human.

PubMed:30707959

[IF=2.656] Fang et al. Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma. (2018) Onco.Targets.Ther. 11:5647-5655 WB ; Human.

PubMed:30254457

[IF=1.829] Zhong Y et al. Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines.Stem Cell Res. 2018 Jul;30:100-112. ICF ; Mouse.

PubMed:29843002

[IF=4.43] Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093 WB ; Human.

PubMed:28167136

[IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human.

PubMed:27824118

[IF=2.98] Deng et al. PEG10 plays a crucial role in human lung cancer proliferation, progression, prognosis and metastasis. (2014) Oncol.Rep. 32:2159-67 WB ; Human.

PubMed:25199998

[IF=1.238] Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135 WB ; Human.

PubMed:28764685

[IF=8.332] Short et al. Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions. (2016) Eur.Respir.. 47:954-66 IF(ICC) ; Human.

PubMed:2674396

[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human.

PubMed:28887916

[IF=3.23] Borin, Thaiz F., et al. "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." PLoS One 12.6 (2017). WB, IHSLCP ; Mouse.

PubMed:28609459

[IF=2.25] Neelam, Sudha, Morgan M. Brooks, and Patrick R. Cammarata. "Lenticular cytoprotection, part 2: Link between glycogen synthase kinase-3β, epithelial to mesenchymal transition, and mitochondrial depolarization." (2015) Molecular Vision. Human.

PubMed:25593505

[IF=3.73] Chen, Cheng-Hsien, et al. "MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis." PloS one 9.6 (2014): e91962. WB ; Rat.

PubMed:24919189