Rabbit Anti-ZNF619 antibody

Home > Product > Antibody > Rabbit Anti-ZNF619 antibody

FLJ90764; zinc finger protein 619; ZN619_HUMAN.

Cat:

SL12231R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from Human ZNF619:188-300/560

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MDA-MB-231 Cell (Human) Lysate at 40 ugPrimary: Anti- ZNF619 (SL12231R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 63 kDObserved band size: 63 kDSample: Lovo Cell (Human) Lysate at 40 ugPrimary: Anti- ZNF619 (SL12231R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 63 kDObserved band size: 63 kDTissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ZNF619 Polyclonal Antibody, Unconjugated(SL12231R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the krueppel C2H2-type zinc-finger protein family, ZNF619 (Zinc finger protein 619) is a 560 amino acid nuclear protein that contains ten C2H2-type zinc fingers. The gene encoding ZNF619 maps to human chromosome 3, which is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Function:
ZNF619 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 10 C2H2-type zinc fingers. ZNF619 may be involved in transcriptional regulation.

Subcellular Location:
Nuclear.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 10 C2H2-type zinc fingers.

SWISS:
Q8N2I2

Gene ID:
285267

Database links:

Entrez Gene: 285267 Human

SwissProt: Q8N2I2 Human

Unigene: 407159 Human

Picture

Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti- ZNF619 (SL12231R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ZNF619 Polyclonal Antibody, Unconjugated(SL12231R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall