Themis is a 641 amino acid nuclear and cytoplasmic protein that exists as three alternatively spliced isoforms. Themis belongs to the themis family and interacts with PLC γ1, Emt and GRB2. Acting through T-cell antigen receptor (TCR) signaling and through the regulation of calcium influx and phosphorylation of ERK 1, Themis plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Themis is phosphorylated on Tyr residues quickly after TCR stimulation. The gene that encodes Themis maps to human chromosome 6q22.33. With 170 million base pairs, chromosome 6 comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6.
Function:
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Phosphorylated on Tyr residues quickly after TCR stimulation.
Similarity:
Belongs to the themis family.
SWISS:
Q8N1K5
Gene ID:
387357
Database links:
Entrez Gene: 387357 Human
Entrez Gene: 210757 Mouse
Omim: 61727 Human
SwissProt: Q8N1K5 Human
SwissProt: Q8BGW0 Mouse
Unigene: 661756 Human
Unigene: 123021 Mouse
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Sample:
U937 Cell (Human) Lysate at 30 ug
Primary: Anti-THEMIS (SL12329R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-THEMIS Polyclonal Antibody, Unconjugated(SL12329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-THEMIS Polyclonal Antibody, Unconjugated(SL12329R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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