Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Human SW96 cell lysates
Lane 3: Human TT cell lysates
Lane 4: Human U87MG cell lysates
Lane 5: Human MCF-7 cell lysates
Primary: Anti-GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
U87MG (Human) Cell Lysate at 30 ug
Primary: Anti- GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNT1) Polyclonal Antibody, Unconjugated (SL12334R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|