Rabbit Anti-BAMBI antibody | Sunlong Medical

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BAMBI; BAMBI_HUMAN; BMP and activin membrane bound inhibitor homolog; BMP and activin membrane-bound inhibitor homolog; NMA; Non metastatic gene A protein; Non-metastatic gene A protein; Putative transmembrane protein NMA.

Cat:

SL12418R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human BAMBI:101-200/260<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: K562 (Human) Cell Lysate at 30 ugLane 2: MCF-7 (Human) Cell Lysate at 30 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugLane 4: A549 (Human) Cell Lysate at 30 ugPrimary: Anti-BAMBI (SL12418R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 30 kDSample: RAW264.7 (Mouse) CellLysate at 30 ugPrimary: Anti-BAMBI (SL12418R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 30 kDSample: Testis (Mouse) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugPrimary: Anti-BAMBI (SL12418R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 30 kDTissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(SL12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.

Function:
Negatively regulates TGF-beta signaling.

Subcellular Location:
Membrane.

Tissue Specificity:
High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.

Similarity:
Belongs to the BAMBI family.

SWISS:
Q13145

Gene ID:
25805

Database links:

Entrez Gene: 25805 Human

Entrez Gene: 13610 Mouse

Entrez Gene: 49801 Pig

Entrez Gene: 83837 Rat

Omim: 604444 Human

SwissProt: Q13145 Human

SwissProt: Q9D0L6 Mouse

SwissProt: Q91XN4 Rat

Unigene: 533336 Human

Unigene: 284863 Mouse

Unigene: 25267 Rat

Picture

Sample:
RAW264.7 (Mouse) CellLysate at 30 ug
Primary: Anti-BAMBI (SL12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD

Sample:
Testis (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-BAMBI (SL12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(SL12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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