Rabbit Anti-Brain2 antibody

Home > Product > Antibody > Rabbit Anti-Brain2 antibody

Brain 2; Brain specific homeobox/POU domain protein 2; Brain-2; Brain-specific homeobox/POU domain protein 2; BRN 2; Brn-2; BRN2; N Oct 3; N OCT 3 Gene; Nervous system specific octamer binding transcription factor; Nervous system-specific octamer-binding

Cat:

SL10077R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Brain2/POU3F2:301-400/443

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebral cortex (Mouse) Lysate at 40 ugPrimary: Anti- Brain2 (SL10077R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49kDSample: Cerebrum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti- Brain2 (SL10077R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49 kDBlank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Brain2 antibody(SL10077R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.

Function:
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.

Subunit:
Interacts with PQBP1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed specifically in the neuroectodermal cell lineage.

Similarity:
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

SWISS:
P20265

Gene ID:
5454

Database links:

Entrez Gene: 5454 Human

Entrez Gene: 18992 Mouse

Entrez Gene: 29588 Rat

Omim: 600494 Human

SwissProt: P20265 Human

SwissProt: P3172 Mouse

SwissProt: P56222 Rat

Unigene: 182505 Human

Unigene: 389520 Mouse

Unigene: 82733 Rat

Picture

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Brain2 (SL10077R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Brain2 antibody(SL10077R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Product Feedback Wall