Rabbit Anti-C21orf33 antibody

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Chromosome 21 open reading frame 33; D21S2048E; ES 1; ES1; Es1 protein; ES1 protein homolog mitochondrial; GT335; HES 1; HES1; Human HES1 protein homolog to E.coli and zebrafish ES1 protein; Keio novel protein I; KNP I; KNPH; KNPI; Protein GT335; Protein

Cat:

SL15124R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human C21orf33:101-200/268

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-C21orf33 (SL15124R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kDTissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(SL15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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KNP-I is a ubiquitously expressed mitochondrial protein that belongs to the ES1 family of proteins. It is a highly conserved protein with homologs identified in various species. This conserved nature suggests an important biological role for the KNP-I protein. The gene that encodes KNP-I (C21orf33) is located on human chromosome 21 in close proximity to a region (D21S25) associated with many genetic diseases. Down syndrome (DS), caused by an extra copy of chromosome 21, is the most common manifestation of trisomic chromosomes. It is likely that the overproduction of the C21orf33 gene product plays a role in the pathology of DS, while its chromosomal location suggests its likely involvement in D21S25 related diseases. While expressed in most tissue, highest expression of KNP-I is found in heart and muscle

Subcellular Location:
Mitochondrion (Potential).

Tissue Specificity:
Ubiquitous, but strongly expressed in heartand skeletal muscle.

Similarity:
Belongs to the ES1 family.

SWISS:
P30042

Gene ID:
8209

Database links:

Entrez Gene: 8209 Human

Omim: 601659 Human

SwissProt: P30042 Human

Unigene: 413482 Human

Picture

Tissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(SL15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).

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