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Home > Product > Antibody > Rabbit Anti-C2orf54 antibody
C2orf54; CB054_HUMAN; Chromosome 2 open reading frame 54; Uncharacterized protein C2orf54.
Cat:
SL15153R
Species Reactivity：
Mouse,(predicted: Human,Rat,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human C2orf54:361-447/447
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Tongue(Mouse)Lysate at 40 ugPrimary: Anti-C2orf54(SL15153R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 50kDObserved band size: 50kDSample: Small intestine (Mouse)Lysate at 40 ugPrimary: Anti-C2orf54(SL15153R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 50kDObserved band size: 50kDTissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Phospho-Insulin Receptor Beta (Tyr1185) Polyclonal Antibody, Unconjugated (SL5453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


C2orf54 (chromosome 2 open reading frame 54), also known as FLJ22671, MGC150431 or MGC150432, is a 447 amino acid protein that exists as three alternatively spliced isoforms, which are encoded by a gene located on human chromosome 2q37.3. The second largest human chromosome, chromosome 2 consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alstr鰉 syndrome is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes seen in modern form today in apes.

SWISS:
Q08AI8

Gene ID:
79919

Database links:

Entrez Gene: 79919 Human

Entrez Gene: 71874 Mouse

SwissProt: Q08AI8 Human

SwissProt: Q8CEZ4 Mouse

Unigene: 193745 Human



Picture

Sample: Tongue(Mouse)Lysate at 40 ug
Primary: Anti-C2orf54(SL15153R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Sample: Small intestine (Mouse)Lysate at 40 ug
Primary: Anti-C2orf54(SL15153R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Insulin Receptor Beta (Tyr1185) Polyclonal Antibody, Unconjugated (SL5453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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