Rabbit Anti-Annexin VI antibody

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Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII;

Cat:

SL8997R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Annexin VI:601-673/673

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Heart (Mouse) Lysate at 40 ugPrimary: Anti-Annexin VI (SL8997R )at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 67/74 kDObserved band size: 70 kDProtein: uterus(mouse) lyates at 40ug; Primary: Rabbit Anti- Annexin VI (SL8997R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:67/74 kDObserved band size:67 kDParaformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (SL8997R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype.

Function:
May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

Tissue Specificity:
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
Phosphorylated in response to growth factor stimulation.

Similarity:
Belongs to the annexin family.
Contains 8 annexin repeats.

SWISS:
P08133

Gene ID:
309

Database links:

Entrez Gene: 309 Human

Entrez Gene: 11749 Mouse

Entrez Gene: 79125 Rat

Omim: 114070 Human

SwissProt: P08133 Human

SwissProt: P14824 Mouse

SwissProt: P9637 Rat

Unigene: 412117 Human

Unigene: 265347 Mouse

Unigene: 204053 Rat

Picture

Protein: uterus(mouse) lyates at 40ug;
Primary: Rabbit Anti- Annexin VI (SL8997R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:67/74 kD Observed band size:67 kD

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (SL8997R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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