FHOD3 (formin homology 2 domain containing 3), also known as FHOS2 or KIAA1695, is a 1,422 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one DAD domain, one FH1 domain, one FH2 domain and one GBD/FH3 domain. Expressed in brain, heart and kidney, FHOD3 interacts with intermediate filaments and functions as an Actin-organizing protein that is thought to promote the formation of stress fibers in conjunction with cellular elongation. Multiple isoforms of FHOD3 exist due to alternative splicing events. The gene encoding FHOD3 maps to human chromosome 18, which houses over 300 protein-coding genes and contains nearly 76 million bases. There are a variety of diseases associated with defects in chromosome 18-localized genes, some of which include Trisomy 18 (also known as Edwards syndrome), Niemann-Pick disease, hereditary hemorrhagic telangiectasia, erythropoietic protoporphyria and follicular lymphomas.
Function:
Actin-organizing protein that may cause stress fiber formation together with cell elongation.
Subunit:
Interacts with nestin/NES-based interfilament (IF) (By similarity). Interacts with SQSTM1; isoform 4 threonine phosphorylation disrupts SQSTM1-binding.
Subcellular Location:
Cytoplasm; cytoskeleton. Main part of the protein localizes to actin fibers and the remaining part displays filamentous staining.
Tissue Specificity:
Expressed in the heart, kidney and brain.
Similarity:
Belongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
SWISS:
Q2V2M9
Gene ID:
80206
Database links:
Entrez Gene: 80206 Human
Entrez Gene: 225288 Mouse
Entrez Gene: 291731 Rat
Omim: 609691 Human
SwissProt: Q2V2M9 Human
SwissProt: Q76LL6 Mouse
Unigene: 630884 Human
Unigene: 329322 Mouse
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Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FHOD3 Polyclonal Antibody, Unconjugated(SL13156R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FHOD3 Polyclonal Antibody, Unconjugated(SL13156R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FHOD3 Polyclonal Antibody, Unconjugated(SL13156R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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