Rabbit Anti-GNL2 antibody | Sunlong Medical

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Autoantigen NGP-1; Autoantigen NGP1; DJ423B22.6 (novel nucleolar guanosine 5'-triphosphate binding protein; FLJ40906; GNL2; Guanine nucleotide binding protein-like 2 (nucleolar); HUMAUANTIG; NGP1; NOG2_HUMAN; Nucleolar GTP binding protein 2; Nucleolar GTP

Cat:

SL13471R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human GNL2:1-100/731

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Muscle (Mouse) Lysate at 40 ugPrimary: Anti- GNL2 (SL13471R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 84 kDObserved band size: 84 kDTissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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GNL2 is a nucleolar guanasine-triphosphate binding protein that is ubiquitously expressed at low levels in almost all tissues. GNL2 is involved in the crucial process of trafficking proteins out of the nucleus. Specifically, it is a GTPase that interacts with the 60s preribosomal subunit in the nucleus and facilitates export of the subunit into the cytoplasm. GTPases are responsible for the hydrolysis of GTP by way of a protein region dubbed the G domain. GTPases are often involved in the translocating proteins through membranes gleaning energy for the activity by hydrolizing GTP. GNL2 shares G domain homology and some functionality with nucleostemin (GNL3), another nuclear GTPase. Highest expression of GNL2 is found in testis.

Function:
GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.

Subcellular Location:
Nucleus; nucleolus.

Tissue Specificity:
Ubiquitously expressed at relatively low levels in all human tissues tested, with the highest level of expression in the testes.

Similarity:
Belongs to the MMR1/HSR1 GTP-binding protein family. NOG2 subfamily.
Contains 1 G (guanine nucleotide-binding) domain.

SWISS:
Q13823

Gene ID:
29889

Database links:

Entrez Gene: 29889 Human

Entrez Gene: 230737 Mouse

Entrez Gene: 362593 Rat

Omim: 609365 Human

SwissProt: Q13823 Human

SwissProt: Q99LH1 Mouse

Unigene: 75528 Human

Unigene: 90760 Mouse

Unigene: 18964 Rat

Picture

Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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