HD-PTP is a 1,636 amino acid protein encoded by the human gene PTPN23. HD-PTP belongs to the protein-tyrosine phosphatase family, non-receptor class subfamily. It contains one BRO1 domain, two TPR repeats and one tyrosine-protein phosphatase domain. The SLCterminal region contains the PTP-like domain, whereas the N-terminal region contains the two TPR regions. These regions are homologous to the yeast protein, BRO1, which is involved in the mitogen-activated protein kinase signaling pathway. Similarly, HD-PTP is believed to act as a negative regulator of Ras-mediated mitogenic activity and is phosphorylated upon DNA damage, probably by ATM or ATR. HD-PTP protein is differentially modulated by two angiogenic growth factors. While Vascular Endothelial Growth Factor (VEGF) has no affect on protein levels, Fibroblast Growth Factor-2 (FGF-2) induces HD-PTP degradation via the proteasome system.
Function:
May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
Subunit:
Interacts with GRAP2 and GRB2.
Subcellular Location:
Nucleus. Cytoplasm. Cytoplasmic vesicle. Endosome. Cytoplasm, cytoskeleton, cilium basal body.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Contains 1 BRO1 domain.
Contains 2 TPR repeats.
Contains 1 tyrosine-protein phosphatase domain.
SWISS:
Q9H3S7
Gene ID:
25930
Database links:
Entrez Gene: 25930 Human
Entrez Gene: 104831 Mouse
Entrez Gene: 117552 Rat
Omim: 606584 Human
SwissProt: Q8N6Z5 Human
SwissProt: Q9H3S7 Human
SwissProt: Q6PB44 Mouse
SwissProt: Q8R1Z5 Mouse
SwissProt: O88902 Rat
SwissProt: Q9QZP8 Rat
Unigene: 25524 Human
Unigene: 123884 Mouse
Unigene: 335477 Mouse
Unigene: 54442 Rat
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDPTP) Polyclonal Antibody, Unconjugated (SL15434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDPTP) Polyclonal Antibody, Unconjugated (SL15434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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