Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Subcellular Location:
Cytoplasmic.
SWISS:
P13646
Gene ID:
Pan Cytokeratin
Database links:
CK13:
Entrez Gene: 3860 Human
Omim:
29665 Human
SwissProt: P13646 Human
Unigene:
654550 Human
CK14:
Entrez Gene: 3861 Human
Omim:
29666 Human
SwissProt: P02533 Human
Unigene:
65876 Human
CK16:
Entrez Gene: 3868 Human
Omim: 29667 Human
SwissProt: P08779 Human
Unigene:
655160 Human
CK17:
Entrez Gene: 3872 Human
Omim:
29669 Human
SwissProt: Q04695 Human
Unigene:
2785 Human
CK19:
Entrez Gene: 3176 Human
Omim:
29620 Human
SwissProt: P08727 Human
Unigene:
654568 Human
CK24:
Entrez Gene: 192666 Human
Omim:
607742 Human
SwissProt: Q2M2I5 Human
Unigene:
87383 Human
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Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti- Pan Cytokeratin Polyclonal Antibody, Unconjugated(SL10403R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti- Pan Cytokeratin Polyclonal Antibody, Unconjugated(SL10403R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL10403R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (SL0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nuclei.
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