Rabbit Anti-CHSS2 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-CHSS2 antibody

Chondroitin glucuronyltransferase II; Chondroitin polymerizing factor; Chondroitin sulfate synthase 2; CHPF; CHSY2; CSS2; CHSS2_HUMAN; Glucuronosyl N acetylgalactosaminyl proteoglycan 4 beta N acetylgalactosaminyltransferase II; N acetylgalactosami

Cat:

SL13928R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human CHSS2:51-150/775

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Siha(human) Cell Lysate at 40 ugPrimary: Anti-CHSS2 (SL13928R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDSample: Mcf-7(human) Cell Lysate at 40 ugPrimary: Anti-CHSS2 (SL13928R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CHSS2 Polyclonal Antibody, Unconjugated(SL13928R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

CHSS2 belongs to the chondroitin N-acetylgalactosaminyltransferase family. It has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity and transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. CHSS2 is ubiquitous; highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.

Function:
Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress.

Subunit:
Binds CHSY1. Isoform 1, isoform 2 and isoform 3 interact with PARK2.

Subcellular Location:
Golgi apparatus, golgi stack membrane; Single pass type II membrane protein.

Tissue Specificity:
Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. Isoform 3 is also ubiquitous. Isoform 2 is expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells.

Similarity:
Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

SWISS:
Q8IZ52

Gene ID:
79586

Database links:

Entrez Gene: 79586 Human

Entrez Gene: 74241 Mouse

Entrez Gene: 316533 Rat

Omim: 610405 Human

SwissProt: Q8IZ52 Human

SwissProt: Q6IQX7 Mouse

Picture

Sample: Mcf-7(human) Cell Lysate at 40 ug
Primary: Anti-CHSS2 (SL13928R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CHSS2 Polyclonal Antibody, Unconjugated(SL13928R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall