Rabbit Anti-EXD2 antibody | Sunlong Medical

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4930539P14Rik; C14orf114; C85658; DKFZp781A0133; DKFZp781L15100; Exd2; EXD2_HUMAN; EXDL2; Exonuclease 3''-5'' domain-containing protein 2; Exonuclease 3''-5'' domain-like-containing protein 2; exonuclease 3'-5' domain-like 2; FLJ10738; RGD1311

Cat:

SL14654R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human EXD2:351-621/621

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lung(Mouse)Lysate at 40 ugPrimary: Anti-EXD2(SL14654R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 70kDObserved band size: 56kDSample: HepG2 (Human)Cell Lysate at 40 ugPrimary: Anti-EXD2(SL14654R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 70kDObserved band size: 56kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXD2) Polyclonal Antibody, Unconjugated (SL14654R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

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Sample: Lung(Mouse)Lysate at 40 ug
Primary: Anti-EXD2(SL14654R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 56kD

Sample: HepG2 (Human)Cell Lysate at 40 ug
Primary: Anti-EXD2(SL14654R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 56kD

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXD2) Polyclonal Antibody, Unconjugated (SL14654R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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