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Home > Product > Antibody > Rabbit Anti-RUFY4/FLJ46536 antibody
RUFY4; RUN and FYVE domain containing protein 4.
Cat:
SL16146R
Species Reactivity：
Mouse,Rat,(predicted: Human,Dog,Pig,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human FLJ46536:131-230/372
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4) Polyclonal Antibody, Unconjugated (SL16146R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4/FLJ46536) Polyclonal Antibody, Unconjugated (SL16146R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4/FLJ46536) Polyclonal Antibody, Unconjugated (SL16146R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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Unit:
Price: $
Product PDFs
Datasheet:


Contains 1 FYVE-type zinc finger and 1 RUN domain.

SWISS:
Q6ZNE9

Gene ID:
28536

Database links:

Entrez Gene: 28536 Human

SwissProt: Q6ZNE9 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4) Polyclonal Antibody, Unconjugated (SL16146R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4/FLJ46536) Polyclonal Antibody, Unconjugated (SL16146R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUFY4/FLJ46536) Polyclonal Antibody, Unconjugated (SL16146R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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