Rabbit Anti-IRAK1BP1 antibody

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ActA binding protein 3; AIP70; IKBP1_HUMAN; Interleukin 1 receptor associated kinase 1 binding protein 1; IRAK1-binding protein 1; Signaling molecule that associates with Pelle-like kinase; SIMPL.

Cat:

SL16694R

Species Reactivity：

Human,Mouse,(predicted: Rat,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human IRAK1BP1:51-150/260

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-IRAK1BP1 (SL16694R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDProtein: MCF-7(human) lyates at 40ug;Primary: Rabbit Anti-IRAK1BP1 (SL16694R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:29 kDObserved band size:33 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated(SL16694R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IRAK1BP1 is a component of the IRAK1 dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. It acts by enhancing RELA transcriptional activity.

Function:
Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. similarity). {ECO:0000250}.

Subunit:
Interacts with IRAK1 and RELA. Interacts with HSPA8 and HSPA1 (By similarity). {ECO:0000250}.

Subcellular Location:
Cytoplasmic and Nuclear

Post-translational modifications:
Phosphorylation at Ser-56 and/or Ser-62 is required for full activity. Phosphorylated on at least one of Ser-235, Thr-237, Ser-242 and Thr-247 upon TNF-alpha activation, which favors nuclear translocation (By similarity). {ECO:0000250}.

Similarity:
Belongs to the IRAK1BP1 family. {ECO:0000305}.

SWISS:
Q5VVH5

Gene ID:
134728

Database links:

Entrez Gene: 134728 Human

Entrez Gene: 65099 Mouse

Omim: 615375 Human

SwissProt: Q5VVH5 Human

SwissProt: Q9ESJ7 Mouse

Unigene: 656212 Human

Unigene: 41415 Mouse

Picture

Protein: MCF-7(human) lyates at 40ug;
Primary: Rabbit Anti-IRAK1BP1 (SL16694R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:29 kD Observed band size:33 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated(SL16694R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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