ODCp is a 92 amino acid protein that is expressed in brain and testis. ODCp is a member of the Orn/Lys/Arg decarboxylase class-II family that catalyzes the creation of CO2 and agmatine from L-arginine. Mammalian ODCp differs from the forms expressed in bacteria and plants and shares less than 50% homology with Ornithine decarboxylase (ODC). ODCp is associated with the mitochondrial membrane where excess agmatine can be degraded by the enzyme Agmatinase or bound by the imidazoline recepto. In the brain, the highest levels of ODCp are found in the hypothalamus. Mammalian ODCp is thermally unstable and can be inhibited by Ca2+, Co2+ and polyamines.
Function:
Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity.
Subunit:
Interacts with OAZ1, OAZ2 and OAZ3; the interactions stabilize the complex by inhibiting AZIN2 ubiquitination and degradation. Does not form an heterodimer with ODC1 (By similarity). Monomer. Interaction with OAZ1, OAZ2 and OAZ3; the interactions lead to increased ornithine decarboxylase (ODC)activity and decreased rate of ODC1 degradation.
Subcellular Location:
Nucleus.
Tissue Specificity:
Highly expressed in brain. Also expressed in testis.
Similarity:
Belongs to the Orn/Lys/Arg decarboxylase class-II family.
SWISS:
Q96A70
Gene ID:
113451
Database links:
Entrez Gene: 506961 Cow
Entrez Gene: 478144 Dog
Entrez Gene: 113451 Human
Entrez Gene: 242669 Mouse
Entrez Gene: 366473 Rat
Omim: 608353 Human
SwissProt: Q96A70 Human
SwissProt: Q8BVM4 Mouse
Unigene: 10367 Human
Unigene: 215814 Mouse
Unigene: 50921 Rat
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Sample: Testis (Mouse)Lysate at 40 ug
Primary: Anti-ODCp(SL16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Sample: Raji (Human)Cell Lysate at 40 ug
Primary: Anti-ODCp(SL16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODCp) Polyclonal Antibody, Unconjugated (SL16872R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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