Rabbit Anti-KAZALD1 antibody

Home > Product > Antibody > Rabbit Anti-KAZALD1 antibody

Kazal-type serine protease inhibitor domain-containing protein 1; KAZALD1; KAZD1_HUMAN.

Cat:

SL17082R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human KAZALD1:71-170/304

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: DU145(Human) Cell Lysate at 40 ugPrimary: Anti-KAZALD1 (SL17082R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 30 kDParaformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (SL17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (SL17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

BONO1, also known as KAZALD1 (kazal-type serine protease inhibitor domain-containing protein 1), IGFBP-rP10, FKSG28 or FKSG40, is a 304 amino acid secreted extracellular matrix protein that promotes matrix assembly. BONO1 is expressed in developing bones and odontoblasts in teeth, where it plays a role in osteoblast proliferation during bone formation and regeneration. BONO1 is also expressed at high levels in spleen, and is found at lower levels in lung, skin, urinary bladder, brain, tongue, kidney and large intestine. Existing as two alternatively spliced isoforms, BONO1 contains one Kazal-like domain, an IGFBP N-terminal domain and a single Ig-like C2-type (immunoglobulin-like) domain. The gene encoding BONO1 maps to human chromosome 10, which contains over 800 genes and 135 million nucleotides. Cockayne syndrome, Cockayne syndrome and trisomy 10 are associated with defects in chromosome 10.

Function:
Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly.

Subcellular Location:
Secreted > extracellular space > extracellular matrix.

Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.

SWISS:
Q96I82

Gene ID:
81621

Database links:

Entrez Gene: 81621 Human

SwissProt: Q96I82 Human

Unigene: 534859 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (SL17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAZALD1) Polyclonal Antibody, Unconjugated (SL17082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall