Home > Product > Antibody > Rabbit Anti-KBTBD11 antibody
Chronic myelogenous leukemia-associated protein; CMLAP; KBTBB_HUMAN; Kbtbd11; Kelch domain-containing protein 7B; kelch repeat and BTB (POZ) domain containing 11; Kelch repeat and BTB domain-containing protein 11; KIAA0711; KLHDC7C.
Cat:
SL17084R
Species Reactivity:
Human,Mouse,(predicted: Rat,Cow,)
Immunogen:
KLH conjugated synthetic peptide derived from human KBTBD11:121-220/623
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: U937(Human) Cell Lysate at 30 ugPrimary: Anti- KBTBD11 (SL17084R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 66 kDObserved band size: 63 kDParaformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (SL17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (SL17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
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Product PDFs
Datasheet:


The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. The Kelch repeat and BTB domain-containing protein 11 (KBTBD11) contains 1 BTB (POZ) domain and 4 Kelch repeats suggesting a role in transcription regulation. The gene encoding KBTBD11 maps to chromosome 8, which contains about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas.

Similarity:
Contains 1 BTB (POZ) domain.
Contains 4 Kelch repeats.

SWISS:
O94819

Gene ID:
9920

Database links:

Entrez Gene: 9920 Human

Entrez Gene: 74901 Mouse

Entrez Gene: 306617 Rat

SwissProt: O94819 Human

SwissProt: Q8BNW9 Mouse

Unigene: 5333 Human

Unigene: 46675 Mouse



Picture

Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- KBTBD11 (SL17084R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (SL17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (SL17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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